Effect of dsDNA on the assembly pathway and mechanical strength of SV40-VP1 virus-like particles

Simian Virus 40 (SV40) is a possible vehicle for targeted drug delivery systems because of its low immunogenicity, high infectivity, and high transfection efficiency. To use SV40 for biotechnology applications, more information is needed on its assembly process to efficiently incorporate foreign materials and to tune the mechanical properties of the structure. We use atomic force microscopy (AFM) to determine the effect of dsDNA packaging, buffer conditions, and incubation time on the morphology and strength of virus-like particles (VLPs) composed of SV40 VP1 pentamers.

https://ift.tt/2Qo2gAB