Bioactive and biocontrol potential of endophytic fungi associated with Brugmansia aurea Lagerh.

Abstract

The present study describes 32 fungal endophytes isolated from different tissues of Brugmansia aurea Lagerh. Each fungal strain was authenticated based on ITS rDNA sequence. Phylogenetic analysis showed that these fungi are distributed in 3 classes, 7 orders and 12 genera. The dichloromethane extracts of endophytic strains were screened for anticancer and antimicrobial activity. Anticancer activity of extracts against human cancer cell lines revealed that fifty % strains are active with IC50 < 10 μg/mL. While analysing antimicrobial potential against both gram positive and gram negative bacteria, 56.25% endophytic strains displayed activity at least against one of the tested human pathogenic bacteria with minimum inhibitory concentration of 12.5–100 μg/mL. In vitro antagonistic activity of endophytes was analysed against Sclerotinia sp., Aspergillus fumigatus, Fusarium solani, Aspergillus flavus and Fusarium oxysporum pathogen. The broad spectrum anti-phytopathogenic activity was shown by R2BA. Presence of KS domain of PKS gene and high degree of bioactivity shown by endophytic fungi suggested that they have potential to produce therapeutic compounds and to serve as biocontrol agent.

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