Abstract
Confocal laser endomicroscopy (CLE)1) and endocytoscopy system (ECS)2) have made optical biopsy in the digestive tract a reality. However, for omission of biopsy histology, visualization of nuclei is essential. In ECS, nuclei can be observed after vital staining with methylene blue, toluidine blue etc.3), and vital staining in CLE requires venous injection or spraying of fluorescein into the gastrointestinal lumen4). However, fluorescein does not stain nuclei. Acriflavin, a topical antiseptic drug, can stain nuclei for CLE observation5), but it has not been widely used because of associated adverse events.
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