A decision tree-based combination of ezrin-interacting proteins to estimate the prognostic risk of patients with esophageal squamous cell carcinoma

Our previous studies have highlighted the importance of ezrin in esophageal squamous cell carcinoma (ESCC). Here our objective was to explore the clinical significance of ezrin-interacting proteins, which would provide a theoretical basis for understanding the function of ezrin and potential therapeutic targets for ESCC. We employed affinity purification and mass spectrometry to identify PDIA3, CNPY2 and STMN1 as potential ezrin-interacting proteins. Confocal microscopy and co-immunoprecipitation analysis further confirmed the colocalization and interaction of ezrin with PDIA3, CNPY2 and STMN1.

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