Publication date: 19 April 2016
Source:Vaccine, Volume 34, Issue 18
Author(s): Chun-Ting Yuen, Catpagavalli Asokanathan, Sarah Cook, Naomi Lin, Dorothy Xing
Pertussis toxin (PTx) is a major virulence factor produced by Bordetella pertussis and its detoxified form is one of the major protective antigens in vaccines against whooping cough. Ideally, PTx in the vaccine should be completely detoxified while still preserving immunogenicity. However, this may not always be the case. Due to multilevel reaction mechanisms of chemical detoxification that act on different molecular sites and with different production processes, it is difficult to define a molecular characteristic of a pertussis toxoid. PTx has two functional distinctive domains: the ADP-ribosyltransferase enzymatic subunit S1 (A-protomer) and the host cell binding carbohydrate-binding subunits S2–5 (B-oligomer); and in this study, we investigated the effect of different detoxification processes on these two functional activities of the residual PTx in toxoids and vaccines currently marketed worldwide using a recently developed in vitro biochemical assay system. The patho-physiological activities in these samples were also estimated using the in vivo official histamine sensitisation tests. Different types of vaccines, detoxified by formaldehyde, glutaraldehyde or by both, have different residual functional and individual baseline activities. Of the vaccines tested, PT toxoid detoxified by formaldehyde had the lowest residual PTx ADP-ribosyltransferase activity. The carbohydrate binding results detected by anti-PTx polyclonal (pAb) and anti-PTx subunits monoclonal antibodies (mAb) showed specific binding profiles for toxoids and vaccines produced from different detoxification methods. In addition, we also demonstrated that using pAb or mAb S2/3 as detection antibodies would give a better differential difference between these vaccine lots than using mAbs S1 or S4. In summary, we showed for the first time that by measuring the activities of the two functional domains of PTx, we could characterise pertussis toxoids prepared from different chemical detoxification methods and this study also highlights the potential use of this in vitro biochemical assay system for in-process control.
from #Medicine via ola Kala on Inoreader http://ift.tt/1RBqWxe
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
-
▼
Μαρτίου
(699)
-
▼
Μαρ 25
(50)
- Broad-Specificity Chemiluminescence Enzyme Immunoa...
- Intending to forget is not easy: Behavioral and el...
- Effectiveness of three pneumococcal conjugate vacc...
- Role of microsatellite instability, immunohistoche...
- Protein S100B: from cancer diagnostics to the eval...
- Automated CH50 liposome-based immunoassay: conside...
- Neutrophil Isolation and Analysis to Determine the...
- A rare case of self-injection of elemental mercury
- Effectiveness of three pneumococcal conjugate vacc...
- Comparable immune responsiveness but increased rea...
- Current status of synthetic hemozoin adjuvant: A p...
- Editorial Board/Aims and Scope
- Hepatitis B vaccination uptake and correlates of s...
- Monitoring the process of measles elimination by s...
- Safety of classical swine fever virus vaccine stra...
- Effect of different detoxification procedures on t...
- An adenoviral cancer vaccine co-encoding a tumor a...
- Editorial Board/Aims and Scope
- Response to Bogaert
- Reply to Brundage and Shanks
- Use of allicin as feed additive to enhance vaccina...
- Memory T cell responses targeting the SARS coronav...
- Dementia and Physical Activity (DAPA) - an exercis...
- The use of paclitaxel coated balloon (PCB) in acut...
- A Karnaugh map based approach towards systemic rev...
- The administration of high-dose propofol sedation ...
- Field homogeneity improvement of maglev NdFeB magn...
- Differential inequalities imposed by the extended ...
- Supramolecular Affinity Chromatography for Methyla...
- Promising effects of treatment with flotation-REST...
- Immune thrombocytopenia after bee venom therapy: a...
- Imaging CD4 T Cell Interstitial Migration in the I...
- Induction of Maternal Immune Activation in Mice at...
- Synthesis, and anticonvulsant activity of new amid...
- 1,2,4-Triazolyl octahydropyrrolo[2,3-b]pyrroles: A...
- Novel nicotine analogues with potential anti-mycob...
- Editorial board
- Isatin analogs as novel inhibitors of Candida spp....
- Antiretroviral (HIV-1) activity of azulene derivat...
- 3D microwave tomography of the breast using prior ...
- Design, synthesis, topoisomerase I & II inhibitory...
- Technical Note: Cortical thickness and density est...
- Calibration of coronary calcium scores determined ...
- Simulation of pseudo-CT images based on deformable...
- Personal exposure to ultrafine particles from PVC ...
- Caveolin-1protects against hepatic ischemia/reperf...
- Exercise training as a drug to treat age associate...
- Synthesis, Evaluation, and Metabolism of Novel [6]...
- A SIRT3/AMPK/autophagy network orchestrates the pr...
- Bayesian Integration and Classification of Composi...
-
▼
Μαρ 25
(50)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.