Invasive fungal infection is a well-known cause of morbidity and mortality in immunocompromised patients. In this study we aimed to evaluate the hepatotoxicity induced by combined therapy of flucytosine and amphotericin B, at three different doses administered to mice for 14 days: 50 mg/kg flucytosine and 300 μg/kg amphotericin B; 100 mg/kg flucytosine and 600 μg/kg amphotericin B; 150 mg/kg flucytosine and 900 μg/kg amphotericin B. Liver injuries were evaluated by analysis of optic and electron microscopy samples, changes in TNF-α, IL-6, and NF-κB inflammation markers levels of expression, and evaluation of mRNA profiles. Histological and ultrastructural analysis revealed an increase in parenchymal and portal inflammation in mice and Kupffer cells activation. Combined antifungal treatment stimulated activation of an inflammatory pathway, demonstrated by a significant dose-dependent increase of TNF-α and IL-6 immunoreactivity, together with mRNA upregulation. Also, NF-κB was activated, as suggested by the high levels found in hepatic tissue and upregulation of target genes. Our results suggest that antifungal combined therapy exerts a synergistic inflammatory activation in a dose-dependent manner, through NF-κB pathway, which promotes an inflammatory cascade during inflammation. The use of combined antifungal therapy needs to be dose limiting due to the associated risk of liver injury, especially for those patients with hepatic dysfunction.
from #Medicine via ola Kala on Inoreader http://ift.tt/1SBG1mP
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
-
▼
Φεβρουαρίου
(793)
-
▼
Φεβ 02
(50)
- A needs assessment of people living with diabetes ...
- Research participation after terrorism: an open co...
- Quantification of low molecular weight selenium me...
- GC-induced tethered repression need GR SUMOylation...
- GC-induced direct repression needs GR SUMOylation ...
- Detection of patterns in sound sequences [Neurosci...
- Spectral structure of intrinsic networks [Neurosci...
- A slow growth bacterial transcription factor [Micr...
- Subunit arrangement in a GluClR [Physiology]
- Correction for Sohl et al., Probing the structural...
- Correction to Supporting Information for Rosenzwei...
- Correction for Ling et al., Extremely high genetic...
- Correction for Ogasawara et al., Rapid and profoun...
- Social regulation of gene expression in zebrafish ...
- Latent EBV sensing via exosomal small RNA transfer...
- T-cell-intrinsic Tif1{alpha}/Trim24 regulates alle...
- MINCR is a MYC-induced lncRNA [Biological Sciences]
- TCF7L1 in HP axis development [Developmental Biology]
- Structure of NDP-forming Ac-CoA synthetase [Bioche...
- Seafood Consumption, Mercury, and Brain Neuropatho...
- National Cluster-Randomized Trial of Duty-Hour Fle...
- Establishment of Genome-edited Human Pluripotent S...
- Stem cell-like Xenopus Embryonic Explants to Study...
- Successful diagnosis of intrahepatic splenosis mim...
- Toward Optimum Benefit-Risk and Reduced Access Lag...
- Distribution patterns of stone-tool reduction: Est...
- La duplication rectale de l’adulte : une cause exc...
- Place de la coloscopie dans la prévention et le dé...
- Facteurs de risque d’hémorragies digestives par ru...
- Dual-Frequency Alternating Current Designer Wavefo...
- Detection of Borrelia burgdorferi sensu lato and s...
- Identification and characterization of Theileria o...
- An updated distribution and hosts: trombiculid mit...
- Antenatal immunisation intentions of expectant par...
- Editorial Board/Aims and Scope
- Clinical experience with the meningococcal B vacci...
- Impact of a publicly funded monovalent rotavirus v...
- Can thermostable vaccines help address cold-chain ...
- A trivalent vaccine candidate against hepatitis E ...
- Intradermal vaccination with un-adjuvanted sub-uni...
- Adverse events following immunization in patients ...
- Infant vaccination timing: Beyond traditional cove...
- Evaluation of the non-toxic mutant of the diphther...
- Immunogenicity and efficacy of alphavirus-derived ...
- The Use of Aspiration Catheter Systems for Embolic...
- Recombinant Brain Natriuretic Peptide for the Prev...
- Development of a Murine Infection Model with Leish...
- The Synthesis and Evaluations of the 68Ga-Lissamin...
- Evaluation of Hepatotoxicity with Treatment Doses ...
- Clinical Effect and Mechanism of Yisui Shengxue Gr...
-
▼
Φεβ 02
(50)
Αναζήτηση αυτού του ιστολογίου
Τρίτη 2 Φεβρουαρίου 2016
Evaluation of Hepatotoxicity with Treatment Doses of Flucytosine and Amphotericin B for Invasive Fungal Infections
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.