Natural products are complex matrices of compounds that are prone to interfere with the label-dependent methods that are typically used for cytotoxicity screenings. Here, we developed a label-free Electric Cell-substrate Impedance Sensing (ECIS)-based cytotoxicity assay that can be applied in the assessment of the cytotoxicity of natural extracts. The conditions to measure the impedance using ECIS were first optimized in mice immortalized hypothalamic neurons GT1-7 cells. The performance of four natural extracts when tested using three conventional cytotoxicity assays in GT1-7 cells, was studied. Betula pendula (silver birch tree) was found to interfere with all of the cytotoxicity assays in which labels were applied. The silver birch extract was also proven to be cytotoxic and, thus, served as a proof-of-concept for the use of ECIS. The extract was fractionated and the ECIS method permitted the distinction of specific kinetic patterns of cytotoxicity on the fractions as well as the extract's pure constituents. This study offers evidence that ECIS is an excellent tool for real-time monitoring of the cytotoxicity of complex extracts that are difficult to work with using conventional (label-based) assays. Altogether, it offers a very suitable cytotoxicity-screening assay making the work with natural products less challenging within the drug discovery workflow.
from #Medicine via ola Kala on Inoreader http://ift.tt/1iXdaej
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
-
▼
2015
(2119)
- ► Δεκεμβρίου (940)
-
▼
Νοεμβρίου
(765)
-
▼
Νοε 12
(48)
- OtoRhinoLaryngology & Medicine,New Articles,Novemb...
- Reply to the Letter “Effect of anti-oxidant agents...
- Age-related effects on verbal and visuospatial mem...
- Molecules, Vol. 20, Pages 20341-20354: Direct Amin...
- Molecules, Vol. 20, Pages 20334-20340: Triterpenoi...
- Molecules, Vol. 20, Pages 20320-20333: Enhancement...
- Molecules, Vol. 20, Pages 20312-20319: Cytotoxic A...
- Molecules, Vol. 20, Pages 20355-20380: α-Linolenic...
- IJMS, Vol. 16, Pages 27072-27086: A Comparative St...
- IJMS, Vol. 16, Pages 27058-27071: Benzene-Induced ...
- IJMS, Vol. 16, Pages 27044-27057: Online Measureme...
- A predictive coding account of MMN reduction in sc...
- Successful cutting balloon angioplasty in a child ...
- Development of repeatable arrays of proteins using...
- Materials, Vol. 8, Pages 7650-7662: High Temperatu...
- Microorganisms, Vol. 3, Pages 866-889: Hydrogen Su...
- Microorganisms, Vol. 3, Pages 851-865: Bioactiviti...
- Molecules, Vol. 20, Pages 20297-20311: Derinat Pro...
- Materials, Vol. 8, Pages 7663-7672: Enhanced Erbiu...
- Prenatal diagnosis of single gene disorders using ...
- Identification of biomarkers to measure HIV-specif...
- Dynamics and role of antibodies to Plasmodium falc...
- Metals, Vol. 5, Pages 2119-2127: Liquid Phase Sepa...
- Metals, Vol. 5, Pages 2109-2118: Dynamic Strain Ev...
- Life, Vol. 5, Pages 1610-1628: Non-Standard Geneti...
- Life, Vol. 5, Pages 1587-1609: Starvation-Survival...
- Laws, Vol. 4, Pages 709-728: The Convention for th...
- Molecules, Vol. 20, Pages 20286-20296: β–Cyclodext...
- Effect of anti-oxidant agents in patients with hep...
- Healthcare, Vol. 3, Pages 1158-1173: The Applicati...
- Games, Vol. 6, Pages 604-636: The Role of Implicit...
- Entropy, Vol. 17, Pages 7680-7697: Using Expectati...
- Forests, Vol. 6, Pages 4088-4104: Characterization...
- Coatings, Vol. 5, Pages 830-864: The Search for Du...
- Coatings, Vol. 5, Pages 816-829: Natural Deposit C...
- Catalysts, Vol. 5, Pages 1928-1947: Molybdenum (VI...
- Catalysts, Vol. 5, Pages 1911-1927: Zr-SBA-15 Lewi...
- Catalysts, Vol. 5, Pages 1897-1910: Direct Convers...
- Biomolecules, Vol. 5, Pages 3142-3176: Immune Mech...
- Microarrays, Vol. 4, Pages 551-569: SNPs Array Kar...
- Applied Sciences, Vol. 5, Pages 1134-1163: An Effi...
- JDB, Vol. 3, Pages 93-111: Pbx4 is Required for th...
- Reproductive history and risk of cognitive impairm...
- Toxins, Vol. 7, Pages 4706-4729: Metabolism of Deo...
- Reasons & Symptoms of Dengue Fever
- Intra-individual lap time variation of the 400-m w...
- IJMS, Vol. 16, Pages 27032-27043: A PPO Promoter f...
- IJMS, Vol. 16, Pages 27015-27031: Sildenafil Prote...
-
▼
Νοε 12
(48)
Αναζήτηση αυτού του ιστολογίου
Πέμπτη 12 Νοεμβρίου 2015
IJMS, Vol. 16, Pages 27044-27057: Online Measurement of Real-Time Cytotoxic Responses Induced by Multi-Component Matrices, such as Natural Products, through Electric Cell-Substrate Impedance Sensing (ECIS)
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.