Carbonic anhydrase inhibitors: design, synthesis, kinetic, docking and molecular dynamics analysis of novel glycine and phenylalanine sulphonamide derivatives

Publication date: Available online 23 October 2015
Source:Bioorganic & Medicinal Chemistry
Author(s): İsmail Fidan, Ramin Ekhteiari Salmas, Mehmet Arslan, Murat Şentürk, Serdar Durdagi, Deniz Ekinci, Esra Şentürk, Sedat Coşgun, Claudiu T. Supuran
The inhibition of two human cytosolic carbonic anhydrase isozymes I and II, with some novel glycine and phenylalanine sulphonamide derivatives were investigated. Newly synthesized compounds G 1-4 and P 1-4 showed effective inhibition profiles with KI values in the range of 14.66 – 315 μM for hCA I and of 18.31 – 143.8 μM against hCA II, respectively. In order to investigate the binding mechanisms of these inhibitors, in silico docking studies were applied. Atomistic Molecular Dynamic simulations were performed for docking poses which utilize to illustrate the inhibition mechanism of used inhibitors into active site of CAII. These sulfonamide containing compounds generally were competitive inhibitors with 4-nitrophenylacetate as substrate. Some investigated compounds here showed effective hCA II inhibitory effects, in the same range as the clinically used sulfonamide, sulfanilamide or mafenide and might be used as leads for generating enzyme inhibitors possibly targeting other CA isoforms which have not been yet assayed for their interactions with such agents.

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