Carcinoembryonic antigen cell adhesion molecule 1 inhibits the antitumor effect of neutrophils in tongue squamous cell carcinoma

Summary

Carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1), a transmembrane glycoprotein, has multiple functions. In tongue squamous cell carcinoma (TSCC), CEACAM1 overexpression is correlated with neutrophil infiltration, and both are associated with poor clinical outcomes. However, the mechanism underlying CEACAM1's effect on neutrophil function in TSCC remains unclear. We cocultured Cal‐27 cells overexpressing CEACAM1 (CEACAM1‐4L, CEACAM1‐4S) and differentiated HL‐60 cells. This significantly upregulated the expression of MMP‐9, interleukin 8, and vascular endothelial growth factor A in the differentiated HL‐60 cells and downregulated the expression of tumor necrosis factor α, relative to vector and blank control groups (P<0.05). Additionally, CEACAM1 overexpression in Cal‐27 cells weakened the cytotoxicity of differentiated HL‐60 cells in the coculture system (P<0.05). Thus, CEACAM1 expression in TSCC may induce an antitumor to protumor transformation of neutrophils. We performed quantitative RT‐PCR and ELISA tests to evaluate the underlying mechanism, and found that CEACAM1 expression in Cal‐27 cells upregulated transforming growth factor β1 (TGF‐β1) expression, while blocking of TGF‐β1 inhibited the neutrophils' changes in the coculture system. Immunohistochemical analysis of clinical specimens revealed strong expression of TGF‐β1 protein in TSCC. TGF‐β1 expression was positively correlated with CEACAM1 expression, lymph node metastasis, and tumor recurrence. Double immunofluorescence results revealed colocalization of CEACAM1 and TGF‐β1 protein in TSCC; a xenograft nude mouse model revealed that CEACAM1 overexpression in TSCC promoted tumor formation and growth. Our results indicate that CEACAM1 overexpression in TSCC may induce transformation of neutrophils from antitumor to protumor type via TGF‐β1, which may further promote tumor progression.

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