Presence of osteocalcin, osteopontin, and reactive oxygen species‐positive cells in pulp tissue after dental bleaching

Abstract

Aim

To analyse the influence of H₂O₂ on pulp repair through osteocalcin and osteopontin immunolabelling, and in cellular defence by using the anti‐reactive oxygen species (ROS) antibody.

Methodology

The maxillary molars of 50 rats were treated with 35% H₂O₂ (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15, and 30 days (n=10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS‐positive cells were counted. Paired t‐test and Wilcoxon signed‐rank test were used (P<0.05).

Results

The Ble group had necrosis in the coronal pulp at 0 h, and in the occlusal third of the coronal pulp at 2 days; at 7, 15, and 30 days, no inflammation was noted similar to the controls (P>0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days, and increased thereafter, differing from the controls at all two periods (P<0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P<0.05). The Ble group had more ROS‐positive cells in the pulp at 7 and 15 days (P<0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P<0.05).

Conclusions

Post‐bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participate in this process, and anti‐ROS was involved in cellular defence against H₂O₂.

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