Ketoconazole and Posaconazole Selectively Target HK2 Expressing Glioblastoma Cells

Purpose: HK2 is elevated in GBM and we have shown that HK2 could serve as an effective therapeutic target for GBM. Here, we interrogated compounds that target HK2 effectively and restrict tumor growth in cell lines, patient derived glioma stem cells, and mouse models of GBM. Experimental Design: We performed a screen using a set of 15 drugs that were predicted to inhibit the HK2 associated gene signature. We next determined the EC₅₀ of the compounds by treating glioma cell lines and glioma stem cells. Selected compounds showing significant impact in vitro were used to treat mice and examine their effect on survival and tumor characteristics. The effect of compounds on tumor cell metabolic activity was also assessed in vitro. Results: This screen identified the azole class of antifungals as inhibitors of tumor metabolism. Among the compounds tested, Ketoconazole and Posaconazole displayed the greatest inhibitory effect on GBM both in vitro and in vivo. Treatment of mice bearing GBM with Ketoconazole and Posaconazole increased their survival, reduced tumor cell proliferation, and decreased tumor metabolism. Additionally, treatment with azoles resulted in increased proportion of apoptotic cells. Conclusions: Overall, we provide evidence that azoles exert their effect by targeting genes and pathways regulated by HK2. These findings shed light on the action of azoles in GBM., Combined with existing literature and pre-clinical results these data support the value of repurposing azoles in GBM clinical trials.

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