Lipid Structure and Composition Control Consequences of Interleaflet Coupling in Asymmetric Vesicles

Using FRET, raft/liquid ordered domain formation was assessed in asymmetric vesicles containing outer leaflets composed of high-Tm saturated phosphatidylcholines (diC18:0PC, diC16:0PC, diC15:0PC or diC14:0PC), low-Tm unsaturated dioleoylphosphatidylcholine (DOPC) and cholesterol, and inner leaflets composed of lipids which by themselves would not form ordered domains, DOPC and cholesterol. Ordered domain formation in the outer leaflet was compared to that in symmetric vesicles with the same lipid composition as the asymmetric vesicle outer leaflets.

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