Spatially Organized β-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans

Understanding how heterogeneous cells within a multicellular system interact and affect overall function is difficult without a means of perturbing individual cells or subpopulations. Here we apply optogenetics to understand how subpopulations of β-cells control the overall [Ca2+]i response and insulin secretion dynamics of the islets of Langerhans. We spatiotemporally perturbed electrical activity in β-cells of channelrhodopsin2-expressing islets, mapped the [Ca2+]i response, and correlated this with the cellular metabolic activity and an in silico electrophysiology model.

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