Publication date: Available online 29 January 2016
Source:Bioorganic & Medicinal Chemistry
Author(s): Muthian Shanmugasundaram, Irudaya Charles, Anilkumar R. Kore
The first example of the synthesis of new dinucleotide cap analog containing propargyl group such as m7,3'-O-propargylG[5']ppp[5']G is reported. The effect of propargyl cap analog with standard cap was evaluated with respect to their capping efficiency, in vitro T7 RNA polymerase transcription efficiency, and translation activity using cultured HeLa cells. It is noteworthy that propargyl cap analog outperforms standard cap by 3.1 fold in terms of translational properties. The propargyl cap analog forms a more stable complex with translation initiation factor eIF4E based on the molecular modeling studies.
Graphical abstract
from #Medicine via ola Kala on Inoreader http://ift.tt/1KfbKZG
via IFTTT
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.