Publication date: Available online 1 November 2015
Source:Vaccine
Author(s): Guomin Wang, Ruihao Zhu, Licong Yang, Kaile Wang, Qian Zhang, Xia Su, Bing Yang, Jue Zhang, Jing Fang
Vaccines are of great importance in controlling the spread of infectious diseases in poultry farming. The safety and efficacy of vaccines are also essential. To explore the feasibility of a novel technology (non-thermal plasma) in inactivated vaccine preparation, an alternating current atmospheric pressure non-thermal plasma (NTP) jet with Ar/O2/N2 as the operating gas was used to inactivate a Newcastle disease virus (NDV, LaSota) strain and H9N2 avian influenza virus (AIV, A/Chicken/Hebei/WD/98) for vaccine preparation. The results showed that complete inactivation could be achieved with 2min of NTP treatment for both NDV and AIV. Moreover, a proper NTP treatment time is needed for inactivation of a virus without destruction of the antigenic determinants. Compared to traditional formaldehyde-inactivated vaccine, the vaccine made from NDV treated by NTP for 2min (NTP-2min-NDV-vaccine) could induce a higher NDV-specific antibody titer in specific pathogen-free (SPF) chickens, and the results of a chicken challenge experiment showed that NTP-2min-NDV-vaccine could protect SPF chickens from a lethal NDV challenge. Vaccines made from AIV treated by NTP for 2min (NTP-2min-AIV-vaccine) also showed a similar AIV-specific antibody titer compared with traditional AIV vaccines prepared using formaldehyde inactivation. Studies of the morphological changes of the virus, chemical analysis of NDV allantoic fluid and optical emission spectrum analysis of NTP suggested that reactive oxygen species and reactive nitrogen species produced by NTP played an important role in the virus inactivation process. All of these results demonstrated that it could be feasible to use non-thermal NTP as an alternative strategy to prepare inactivated vaccines for Newcastle disease and avian influenza.
from #Medicine via ola Kala on Inoreader http://ift.tt/1LLk6FQ
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
-
▼
2015
(2119)
- ► Δεκεμβρίου (940)
-
▼
Νοεμβρίου
(765)
-
▼
Νοε 01
(31)
- Therapeutic effects of inorganic nitrate and nitri...
- Intake of Meals Containing High Levels of Carbohyd...
- Production of high titer attenuated poliovirus str...
- Pediatric provider vaccine hesitancy: An under-rec...
- Non-thermal plasma for inactivated-vaccine prepara...
- Assessment of the enhancement of PLGA nanoparticle...
- Intranasal immunisation with recombinant adenoviru...
- Randomized comparison of the safety of Flublok® ve...
- Incidence of invasive pneumococcal disease in 5–15...
- The bm12 Inducible Model of Systemic Lupus Erythem...
- Strength training, but not endurance training, red...
- Factors to consider in identifying critical points...
- Nanoparticle Probes for Structural and Functional ...
- Reducing sitting time after stroke. A Phase II saf...
- A Cross-Sectional Examination of the Associations ...
- Isoflurane Damages the Developing Brain of Mice an...
- Prognostic Significance of the Tumor-Stroma Ratio ...
- Effectiveness and Safety of Manufactured Chinese H...
- The Combination of Resveratrol and Quercetin Atten...
- MR Prediction of Liver Function and Pathology Usin...
- Magnetic Resonance Imaging as a Biomarker for Rena...
- Endogenous Ouabain: An Old Cardiotonic Steroid as ...
- Novel Sensor-Enabled Ex Vivo Bioreactor: A New App...
- Coevolution between Cancer Activities and Food Str...
- Upconversion nanosensor for sensitive fluorescence...
- Metabolomic profile related to cardiovascular dise...
- Synthesis and Biological Profiling of 6- or 7-(Het...
- Synthesis and Evaluation of 3-Hydroxy-3-phenylprop...
- Big Data and Network Biology 2015
- Synergistic Effects of BMP9 and miR-548d-5p on Pro...
- Interoceptive awareness in patients with functiona...
-
▼
Νοε 01
(31)
Αναζήτηση αυτού του ιστολογίου
Κυριακή 1 Νοεμβρίου 2015
Non-thermal plasma for inactivated-vaccine preparation
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.