IGFBP5 is an important regulatory protein in breast cancer progression. We tried to identify differentially expressed genes (DEGs) between breast tumor tissues with IGFBP5 overexpression and their adjacent normal tissues. In this study, thirty-eight breast cancer and adjacent normal breast tissue samples were used to determine IGFBP5 expression by qPCR. cDNA microarrays were applied to the highest IGFBP5 overexpressed tumor samples compared to their adjacent normal breast tissue. Microarray analysis revealed that a total of 186 genes were differentially expressed in breast cancer compared with normal breast tissues. Of the 186 genes, 169 genes were downregulated and 17 genes were upregulated in the tumor samples. KEGG pathway analyses showed that protein digestion and absorption, focal adhesion, salivary secretion, drug metabolism-cytochrome P450, and phenylalanine metabolism pathways are involved. Among these DEGs, the prominent top two genes (MMP11 and COL1A1) which potentially correlated with IGFBP5 were selected for validation using real time RT-qPCR. Only COL1A1 expression showed a consistent upregulation with IGFBP5 expression and COL1A1 and MMP11 were significantly positively correlated. We concluded that the discovery of coordinately expressed genes related with IGFBP5 might contribute to understanding of the molecular mechanism of the function of IGFBP5 in breast cancer. Further functional studies on DEGs and association with IGFBP5 may identify novel biomarkers for clinical applications in breast cancer.
from #Medicine via ola Kala on Inoreader http://ift.tt/1QfsPnE
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
-
▼
2015
(2119)
- ► Δεκεμβρίου (940)
-
▼
Νοεμβρίου
(765)
-
▼
Νοε 10
(50)
- Sensors, Vol. 15, Pages 28271-28286: Real-Valued C...
- Remote Sensing, Vol. 7, Pages 14988-15013: A Compa...
- A qualitative evidence synthesis on the management...
- Adherence to competing strategies for colorectal c...
- Lateral approach liver hanging maneuver in laparos...
- Meta-analysis of laparoscopic surgery for recurren...
- Identification of novel anti-hepatitis C virus age...
- Angiogenesis and vascular endothelial growth facto...
- Dexmedetomidine versus morphine infusion following...
- Tripterygium wilfordii hook F as maintenance treat...
- Variation in treatment priorities for chronic hepa...
- Randomized comparison of surveillance intervals in...
- Positive regulation of β-catenin–PROX1 signaling a...
- Prognostic factors for progression-free and overal...
- Prospective cohort study comparing transient EUS g...
- Palliative chemotherapy for patients 70 years of a...
- Influence of the intervention of exercise on obese...
- Characteristics of colonoscopic findings in the ve...
- Changes in insulin sensitivity and secretion after...
- Prevalence of pathogenic mutations in cancer predi...
- Current status of immunomodulatory therapy in chro...
- Blunting of colon contractions in diabetics with g...
- Family physicians’ intention to support women in m...
- Sharp-Hook Acupuncture (Feng Gou Zhen) for Patient...
- Cross-Sectional Study of Respiratory Symptoms, Spi...
- JFMK, Vol. 1, Pages 16-29: Clinical Kinesiology an...
- Insects, Vol. 6, Pages 943-960: Mating Success, Lo...
- Genes, Vol. 6, Pages 1201-1214: Identification of ...
- Graded Resistance Exercise And Type 2 Diabetes in ...
- Effect of a high-dose target-controlled naloxone i...
- Automated analysis of single cells using Laser Twe...
- Information, Vol. 6, Pages 733-749: Digital Inform...
- IJMS, Vol. 16, Pages 26880-26913: Therapeutic Impl...
- IJMS, Vol. 16, Pages 26871-26879: Identification o...
- IJMS, Vol. 16, Pages 26850-26870: Augmenting the A...
- Inorganics, Vol. 3, Pages 500-510: Holmium(III) Su...
- IJMS, Vol. 16, Pages 26880-26913: Therapeutic Impl...
- IJMS, Vol. 16, Pages 26871-26879: Identification o...
- IJMS, Vol. 16, Pages 26850-26870: Augmenting the A...
- IJMS, Vol. 16, Pages 26832-26849: Single-Cell Isol...
- IJERPH, Vol. 12, Pages 14312-14326: Kinetics and M...
- IJERPH, Vol. 12, Pages 14301-14311: Exposure to Ai...
- IJERPH, Vol. 12, Pages 14285-14300: Arsenic and Ot...
- IJMS, Vol. 16, Pages 26832-26849: Single-Cell Isol...
- Drug Delivery Using Novel Biological and Synthetic...
- Exploring Clinical and Epidemiological Characteris...
- Changes of Plantar Pressure and Gait Parameters in...
- Senescence-Associated Changes in Proteome and O-Gl...
- Selection of the Strain Lactobacillus acidophilus ...
- Cancers, Vol. 7, Pages 2262-2276: Differences in R...
-
▼
Νοε 10
(50)
Αναζήτηση αυτού του ιστολογίου
Τρίτη 10 Νοεμβρίου 2015
Genes, Vol. 6, Pages 1201-1214: Identification of Differentially Expressed IGFBP5-Related Genes in Breast Cancer Tumor Tissues Using cDNA Microarray Experiments
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.