Publication date: November 2015
Source:DNA Repair, Volume 35
Author(s): Giulia Antoniali, Federica Marcuzzi, Elena Casarano, Gianluca Tell
Cadmium (Cd) is a carcinogenic and neurotoxic environmental pollutant. Among the proposed mechanisms for Cd toxic effects, its ability to promote oxidative stress and to inhibit, in vitro, the activities of some Base Excision DNA Repair (BER) enzymes, such as hOGG1, XRCC1 and APE1, have been already established. However, the molecular mechanisms at the basis of these processes are largely unknown especially at sub-lethal doses of Cd and no information is available on the effect of Cd on the expression levels of BER enzymes. Here, we show that non-toxic treatment of neuronal cell lines, with pro-mitogenic doses of Cd, promotes a significant time- and dose-dependent down-regulation of DNA polymerase δ (POLD1) expression through a transcriptional mechanism with a modest effect on Polβ, XRCC1 and APE1. We further elucidated that the observed transcriptional repression on Polδ is acted by through competition by activated p53 on Sp1 at POLD1 promoter and by a squelching effect. We further proved the positive effect of Sp1 not only on POLD1 expression but also on Polβ, XRCC1 and APE1 expression, suggesting that Sp1 has pleiotropic effects on the whole BER pathway. Our results indicated that Cd-mediated impairment of BER pathway, besides acting on the enzymatic functions of some key proteins, is also exerted at the gene expression level of Polδ by acting on the p53–Sp1 regulatory axis. These data may explain not only the Cd-induced neurotoxic effects but also the potential carcinogenicity of this heavy metal.
from #Medicine via ola Kala on Inoreader http://ift.tt/1LTo3Zd
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
-
▼
2015
(2119)
- ► Δεκεμβρίου (940)
-
▼
Νοεμβρίου
(765)
-
▼
Νοε 05
(50)
- Editorial Board/Aims and Scope
- Prospective on multiscale simulation of virus-like...
- Soluble Urokinase Receptor and Chronic Kidney Disease
- Influence of Serum and Hypoxia on Incorporation of...
- Dietary ALA from Spinach Enhances Liver n-3 Fatty ...
- Two stages of XRCC1 recruitment and two classes of...
- Modulation of DNA damage responseand induction of ...
- Cadmium treatment suppresses DNA polymerase δ cata...
- Versatility in phospho-dependent molecular recogni...
- Role of mismatch repair proteins in the processing...
- In vitro chromatin templates to study nucleotide e...
- Historical perspective on the DNA damage response
- Evelyn Witkin and the Coordinated Response to DNA ...
- Design, synthesis, and characterization of nucleos...
- Evaluation of leishmanicidal activity and cytotoxi...
- Prevalence of intestinal parasites among patients ...
- Photolithographic Synthesis of High-Density DNA an...
- Tracking the Invasion of Small Numbers of Cells in...
- Elasto-Inertial Pinched Flow Fractionation for Con...
- Conditions Affecting Social Space in Drosophila me...
- Synthesis and Functionalization of 3D Nano-graphen...
- Preventing ICU Subsyndromal Delirium Conversion to...
- Economic Evaluation of Telemedicine for Patients i...
- Comparison Between Neurally Adjusted Ventilatory A...
- A Dysregulated Balance of Proinflammatory and Anti...
- Interleukin-17A Is Associated With Alveolar Inflam...
- Evaluation Following Staggered Implementation of t...
- The Effect of Dexamethasone on Symptoms of Posttra...
- Protective Effect of Areca catechu Leaf Ethanol Ex...
- Serum 25-Hydroxyvitamin D and Osteoarthritis in Ol...
- Total error vs. measurement uncertainty: revolutio...
- Selective extraction and determination of fluoroqu...
- Assessing similarity analysis of chromatographic f...
- Determination of d -serine in human serum by LC-MS...
- Determination of Effective Stability Constants of ...
- Chronic Intermittent Hypoxia and Blood Pressure: I...
- IFN-γ Induces Mimic Extracellular Trap Cell Death ...
- Special Collection: Emerging Concepts in Three-Dim...
- Serum 25-Hydroxyvitamin D and Osteoarthritis in Ol...
- Chronic Intermittent Hypoxia and Blood Pressure: I...
- Dielectric Barrier Discharge Ionization of Perfluo...
- Label-Free and Sensitive Detection of Thrombomodul...
- A J-shaped relationship between caloric intake and...
- Low Resolution Data-Independent Acquisition in an ...
- Video 3. Incorrectly Predicted Transitions During ...
- Video 2. Correctly Predicted Transitions During Re...
- Video 1. Ambulation Mode Transitions Using a Power...
- Ocular Movements, Heel-to-Shin Test, and Gait Asse...
- Simultaneous Electrodialytic Preconcentration and ...
- Surfactant modulated aggregation induced enhanceme...
-
▼
Νοε 05
(50)
Αναζήτηση αυτού του ιστολογίου
Πέμπτη 5 Νοεμβρίου 2015
Cadmium treatment suppresses DNA polymerase δ catalytic subunit gene expression by acting on the p53 and Sp1 regulatory axis
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.