Rapid production of engineered human primary hepatocyte/fibroblast sheets

Publication date: December 2015
Source:Data in Brief, Volume 5
Author(s): Yusuke Sakai, Makiko Koike, Akihiko Soyama, Masaaki Hidaka, Tamotsu Kuroki, Susumu Eguchi
This data article contains data related to the research article entitled "Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice," published in Biomaterials[1]. Engineered hepatocyte/fibroblast sheets (EHFSs) are used for construction of vascularized subcutaneous liver tissue without a pre-transplant vascularization procedures. Here, we described a rapid production technique of EHFSs by controlling fibroblast density and coating fetal bovine serum (FBS) onto temperature-responsive culture dishes (TRCDs). The human fibroblast monolayer formed on FBS-coated TRCDs within 1h when seeded at a high density (at least 1.56×10⁵cells/cm²). The most rapid EHFS production was achieved soon after the adhesion of human primary hepatocytes onto the fibroblast layer.



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