Intervertebral disc (IVD) degeneration is strongly associated with low back pain, a major cause of disability worldwide. An in-depth understanding of IVD cell physiology is required for the design of novel regenerative therapies. Accordingly, aim of this work was the study of IVD cell responses to mitogenic growth factors in a three-dimensional (3D) organotypic milieu, comprising characteristic molecules of IVD's extracellular matrix. In particular, annulus fibrosus (AF) cells were cultured inside collagen type-I gels, while nucleus pulposus (NP) cells in chondroitin sulfate A (CSA) supplemented collagen gels, and the effects of Platelet-Derived Growth Factor (PDGF), basic Fibroblast Growth Factor (bFGF), and Insulin-Like Growth Factor-I (IGF-I) were assessed. All three growth factors stimulated DNA synthesis in both AF and NP 3D cell cultures, with potencies similar to those observed previously in monolayers. CSA supplementation inhibited basal DNA synthesis rates, without affecting the response to growth factors. ERK and Akt were found to be phosphorylated following growth factor stimulation. Blockade of these two signaling pathways using pharmacologic inhibitors significantly, though not completely, inhibited growth factor-induced DNA synthesis. The proposed culture systems may prove useful for further in vitro studies aiming at future interventions for IVD regeneration.
from #Medicine via ola Kala on Inoreader http://ift.tt/1jIm8NV
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
-
▼
2015
(2119)
- ► Δεκεμβρίου (940)
-
▼
Οκτωβρίου
(414)
-
▼
Οκτ 25
(39)
- Instrumentation of Near-term Fetal Sheep for Multi...
- Catecholic amides as potential selective phosphodi...
- Helical peptide–polyamine and –polyether conjugate...
- Bivalent ligands incorporating curcumin and diosge...
- Synthesis and biological evaluation of di-aryl ure...
- Does Computer-Assisted Femur First THR Improve Mus...
- Hyaluronan’s Role in Fibrosis: A Pathogenic Factor...
- Application of Mathematical Modeling for Simulatio...
- Simulation of Ectopic Pacemakers in the Heart: Mul...
- Dental Implants Fatigue as a Possible Failure of I...
- Increased Expression of Serglycin in Specific Carc...
- Vapotage chez les étudiants à l’université et rela...
- Functional and Phenotypic Plasticity of CD4+ T Cel...
- Recent Advances on Pathophysiology, Diagnostic and...
- In Vivo Targeted Magnetic Resonance Imaging of End...
- Revisiting the Posttherapeutic Cure Criterion in C...
- Species Composition and Diversity of Malaria Vecto...
- Organotypic Cultures of Intervertebral Disc Cells:...
- Effects of Fourteen-Day Bed Rest on Trunk Stabiliz...
- The Impact of a Vestibular-Stimulating Exercise Re...
- Effects of Surface Modification and Bulk Geometry ...
- Vitamin A Impairs the Reprogramming of Tregs into ...
- High LIN28A Expressing Ovarian Cancer Cells Secret...
- Simulating Cardiac Electrophysiology Using Unstruc...
- An Overview of the Mechanical Integrity of Dental ...
- T Helper Subsets, Peripheral Plasticity, and the A...
- Effects of Exercise on Spinal Deformities and Qual...
- Cognitive Interventions in Older Persons: Do They ...
- Characterization of Electrograms from Multipolar D...
- A Comprehensive Review on Cyclodextrin-Based Carri...
- Hexosamine-Induced TGF-β Signaling and Osteogenic ...
- Tinnitus and Headache
- The Effect of Risk Factors on the Levels of Chemic...
- Image-Based Structural Modeling of the Cardiac Pur...
- The Association between Cerebral White Matter Lesi...
- Epidemiology of rubella and congenital rubella syn...
- A cytomegalovirus DNA vaccine induces antibodies t...
- Analyse coût-efficacité de l’introduction du vacci...
- Neptunism and Transformism: Robert Jameson and oth...
-
▼
Οκτ 25
(39)
Αναζήτηση αυτού του ιστολογίου
Κυριακή 25 Οκτωβρίου 2015
Organotypic Cultures of Intervertebral Disc Cells: Responses to Growth Factors and Signaling Pathways Involved
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.