Background: Tumor recurrence is a major complication following liver transplantation (LT) as treatment for hepatocellular carcinoma (HCC). Immunosuppression is an important risk factor for HCC recurrence, but conceivably may depend on the type of immunosuppressive medication. Mycophenolic acid (MPA) is a currently widely used immunosuppressant. This study investigated the effects of MPA on HCC. Methods: Three human HCC cell lines and organoids from mouse primary liver tumor were used as experimental models. MTT, Alamar Blue assay, cell cycle analysis, colony formation and [3H]-Thymidine assays were performed. A LT database was used for retrospective analysis of the effect of mycophenolate mofetil (MMF), the prodrug of MPA, on HCC recurrence. Results: With clinically achievable concentrations, MPA effectively inhibited HCC cell proliferation and single cell colony formation unit (CFU). In short-term experiments, MPA effectively elicited S phase arrest in HCC cell lines. In addition, the initiation and growth of liver tumor organoids were effectively inhibited by MPA. Most importantly, the use of MMF in patients with HCC-related LT was significantly associated with less tumor recurrence and improved patient survival. Conclusions: MPA can specifically counteract HCC growth in vitro and tumor recurrence in LT patients. These results warrant prospective clinical trials into the role of MPA-mediated immunosuppression following LT of patients with HCC. # These authors contribute equally Conflicts of interest: The authors declare no conflict of interest. Funding: Supported by the Central Universities deriving from the Northwest Minzu University (No. 31920180124); Zhejiang Provincial Natural Science Foundation of China (No. LY18H160066); Science Foundation of Zhejiang Sci-Tech University (ZSTU) under Grant (No. 17042057-Y); Ministry of Science and Technology Assistance Project Grant (No. KY201501005). Acknowledgment: We thank the Center for Drug Design, University of Minnesota, Minneapolis, USA, for supporting the development of the IMPDH inhibitors. Author Contributions: K.C was responsible for acquisition of data, analysis and interpretation of data and drafting of the manuscript; J.S, B.M, J.L and P.P.C.B performed experiments and data analysis; P.Y.H, D.S and A.S.W.T collected clinical information and performed analysis; K.F and K.P were involved in compound design and synthesis; Z.M, J.K and H.J.M were involved in discussing the project, interpretation of data and critical revision of the manuscript; M.P was involved in analysis and interpretation of data, study supervision and critical revision of the manuscript; Q.P was responsible for study concept and design, analysis and interpretation of data, funding obtain, study supervision and critical revision of the manuscript. *To whom correspondence should be addressed: Qiuwei Pan, Department of Gastroenterology and Hepatology, Erasmus MC, room Na-1005, 'sGravendijkwal 230, NL-3015 CE Rotterdam, The Netherlands, Phone: +31(0)107037502, Fax: +31-(0)107032793. E-mail: q.pan@erasmusmc.nl Copyright © 2019 Wolters Kluwer Health, Inc. All rights reserved.
http://bit.ly/2WYQG2j
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
▼
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
-
▼
Φεβρουαρίου
(5586)
-
▼
Φεβ 11
(270)
- Acquired factor V inhibitor after antibiotic treat...
- A case report of congenital erythropoietic anemia ...
- The nociceptive withdrawal reflex of the trunk is ...
- Propofol and isoflurane on intraoperative motor ev...
- Analgesia nociception index and systemic haemodyna...
- Postoperative analgesic efficacy : Low bolus dose ...
- Altered anatomical relationship between internal j...
- Anaesthetic management of bilateral temporomandibu...
- During general anaesthesia an average adult produc...
- Anaesthesia for tracheal reconstruction
- Displaced paediatric central venous catheter causi...
- Anaesthesia
- Clinical and Preventive Cardiology
- Clinical and Preventive Cardiology
- The Voice Performance After Septoplasty
- Pitch-Synchronous Analysis of Human Voice
- Spontaneous bacterial peritonitis in hepatitis C v...
- Mast cell, a new player in type 2 diabetes
- Does CBD Oil Lower Blood Pressure?
- Kasr Al Ainy Medical Journal
- Prediction of fetal loss in Chinese pregnant patie...
- Risk factors associated with premature myocardial ...
- Perspectives of healthcare professionals in Englan...
- 'When you hear hooves, think zebras, not horses; t...
- Spontaneous pneumomediastinum complicating asthma ...
- Disseminated CMV infection and HLH in a patient wi...
- Anorexia nervosa presenting as diffuse spontaneous...
- Unexpected extremity injury in orthopaedic emergen...
- Rare variant of internal carotid artery anatomy
- When a bruise gets important: Bednar tumour
- Gas-forming gluteal abscess after intramuscular se...
- Canalicular laceration visualisation using chandel...
- One aneurysm and two pseudoaneurysms, same patient
- Hydrochlorothizide-induced acute generalised exant...
- ACUTE LEUKEMIAS XVII Biology and Treatment Strategies
- How Indianapolis EMS improved communications with ...
- Combative patients: Don't be the 'calm down guy'
- Prolonged conservative treatment in patients with ...
- Clinicopathological significance of ataxia telangi...
- Androgen deprivation therapy with chemotherapy or ...
- Breast sensibility after mastectomy and implant-ba...
- Clinical implementation of pre-treatment DPYD geno...
- Systemic treatment and radiotherapy, breast cancer...
- The effect of chemotherapy on subjective cognitive...
- Taming expectations of metformin as a treatment to...
- The Effect of Multispecies Probiotic Supplementati...
- Mitochondrial DNA Fitness Depends on Nuclear Genet...
- Bad to the Bone: The Role of the Insulin-Like Grow...
- A Phase I, Multicenter, Dose-Escalation Study of t...
- 89Zr-labeled Bispecific T-cell Engager AMG 211 PET...
- Evaluation of salivary exosomal chimeric GOLM1-NAA...
- Characterization of hypermutator Pseudomonas aerug...
- Conjugal Transfer, Whole Genome Sequencing, and Pl...
- Activity of a long-acting injectable bedaquiline f...
- In Vitro Evaluation of the Drug Interaction Potent...
- Population Pharmacokinetics of Doravirine and Expo...
- Pharmacokinetic Interactions Between the HCV Inhib...
- Dynamics of resistance plasmids in extended spectr...
- Efficacy and improved resistance potential of a co...
- Select {beta}-lactam combinations exhibit synergy ...
- Pharmacokinetics of albendazole, albendazole sulfo...
- Pharmacokinetics, Safety, and Tolerability of Oxfe...
- AmpI functions as an iron exporter to alleviate {b...
- Optimization of methionyl tRNA-synthetase inhibito...
- Role of Viscoelasticity in Bacterial Killing by An...
- Circulation of plasmids harboring resistance genes...
- Dose Optimization of Colistin Combinations against...
- Population Pharmacokinetic Assessment of Vancomyci...
- In vitro and intracellular activity of imipenem co...
- Bacterial Cytological Profiling (BCP) as a tool to...
- 3-D Imaging Reveals Why Tumors Grow In or Out [New...
- Is my jaw broken or dislocated?
- Peritoneal Surface Oncology in Singapore
- An Educational Review About Using Cost Data for th...
- Drug sensitivity testing on patient-derived sarcom...
- Physical activity and subsequent risk of kidney, b...
- Prognostic impact of CD8 and programmed death-liga...
- Validated clinico-pathologic nomogram in the predi...
- The physiological mTOR complex 1 inhibitor DDIT4 m...
- A multi-national, randomised, open-label, parallel...
- FDA Warns 17 Companies About Illegal Alzheimer Dis...
- Many Children Not Receiving Needed Mental Health T...
- Congo Ebola Outbreak Claims Nearly 100 Children's ...
- Low Fitness, Obesity Linked to Later Disability Pe...
- Racial/Ethnic, Income Variation ID'd in the Opioid...
- Prognostic value of adipose tissue and muscle mass...
- Histomorphometric Analysis of Callus Formation Sti...
- 8 things we wish the general public knew about EMS
- 4 ways to prepare EMTs for violent patients
- Combative patients: Don't be the 'calm down guy'
- Artemether/Lumefantrine for the Treatment of P. ma...
- Vision loss and 12-year risk of dementia in older ...
- Direct comparative effectiveness and safety betwee...
- Patterns of Failure and Survival Outcomes After To...
- Drug sensitivity testing on patient-derived sarcom...
- The physiological mTOR complex 1 inhibitor DDIT4 m...
- Physical activity and subsequent risk of kidney, b...
- A multi-national, randomised, open-label, parallel...
- Validated clinico-pathologic nomogram in the predi...
- Prognostic impact of CD8 and programmed death-liga...
-
▼
Φεβ 11
(270)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.