This study used CCK8 assay, real‐time polymerase chain reaction, Western blot, and flow cytometry to determine the effect of nobiletin in C666‐1 cells. And the result showed that nobiletin induces apoptosis in C666‐1 cells.
Abstract
Background/Aim
Nobiletin, a major polymethoxyflavones (PMFs) from citri reticulatae pericarpium (CRP), can inhibit several forms of cancer proliferation. However, the effects of nobiletin on nasopharyngeal carcinoma (NPC) C666‐1 cells remain largely unknown.
Materials and Methods
Cell counting kit 8 (CCK8) assay was used to measure cell vitality. Flow cytometry was performed to measure the apoptosis rate. Quantitative real‐time polymerase chain reaction (qRT‐PCR) and Western blot analysis were applied to determine the expression of mRNA and protein, respectively.
Results
We showed that the proliferation rate of C666‐1 cells was inhibited and the apoptosis rate was raised after treating with nobiletin. Moreover, nobiletin inhibited the expression of poly(ADP‐ribose)polymerase‐2 (PARP‐2), and the tumor suppression effect of nobiletin on C666‐1 is associated with PARP‐2‐dependent pathway.
Conclusion
We demonstrated for the first time that nobiletin inhibited the growth of C666‐1 cells, which may be relative to its regulation on PARP‐2/SIRT1/AMPK signaling pathway. Our result implied that nobiletin may serve as a strategy to treat nasopharyngeal carcinoma.
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