An IncR Plasmid harbored by a hypervirulent, carbapenem resistant Klebsiella pneumoniae strain possesses five tandem repeats of the blaKPC-2::NTEKPC-Id fragment [Mechanisms of Resistance]

Completed sequences of three plasmids from a carbapenem-resistant, hypervirulent Klebsiella pneumoniae isolate, SH9, were obtained. In addition to the a pLVPK-like virulence-conferring plasmid (pVir-CR-HvKP_SH9), the two MDR plasmids (pKPC-CR-HvKP4_SH9 and pCTX-M-CR-HvKP4_SH9) were predicted to originate from a single pKPC-CR-HvKP4-like multi-replicon plasmid through homologous recombination. Interestingly, bla_KPC-2 gene was detectable in five tandem repeats exhibiting the format of a NTE_KPC-Id-like transposon (IS26-Tn3-ISKpn8-bla_KPC-2-ISKpn6-korC-orf-IS26). The data suggested the important role of DNA recombination on mediating active plasmid evolution.

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