Optical recording of action potential initiation and propagation in mouse skeletal muscle fibers

Skeletal muscle fibers have been used to examine a variety of cellular functions and pathologies. Among other parameters, skeletal muscle action potential propagation has been measured to assess the integrity and function of skeletal muscle. In this paper, we utilize Di-8-ANEPPS, a potentiometric dye, and mag-fluo-4, a low-affinity intracellular Ca2+indicator to non-invasively and reliably measure action potential (AP) conduction velocity in skeletal muscle. We used remote extracellular bipolar electrodes to generate an alternating polarity electric field that initiates an action potential at either end of the fiber.

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