Engineering Cell Surface Function with DNA Origami

Abstract

A specific and reversible method is reported to engineer cell-membrane function by embedding DNA-origami nanodevices onto the cell surface. Robust membrane functionalization across epithelial, mesenchymal, and nonadherent immune cells is achieved with DNA nanoplatforms that enable functions including the construction of higher-order DNA assemblies at the cell surface and programed cell–cell adhesion between homotypic and heterotypic cells via sequence-specific DNA hybridization. It is anticipated that integration of DNA-origami nanodevices can transform the cell membrane into an engineered material that can mimic, manipulate, and measure biophysical and biochemical function within the plasma membrane of living cells.

Cell membranes are engineered by embedding DNA-origami nanodevices onto the surface of live cells. Functionalities including programmed homotypic and heterotypic cell–cell adhesion via sequence-specific DNA hybridization are demonstrated. Embedding DNA-origami nanodevices onto the cell membrane provides a foundation to mimic, control, and monitor biological processes and interactions in local cell environments.

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