Real-Time PCR Targeting penA Mosaic XXXIV Type for Prediction of Extended-Spectrum Cephalosporins Susceptibility in Clinical Neisseria gonorrhoeae Isolates [PublishAheadOfPrint]

N. gonorrhoeae with decreased susceptibility to extended-spectrum cephalosporins (ESCs) are increasing. We developed an assay to predict N. gonorrhoeae susceptibility to ESCs by targeting penA mosaic XXXIV, an allele prevalent among U.S. isolates with elevated ESC MICs. The assay was 97% sensitive and 100% specific in predicting at least one ESC minimum inhibitory concentration (MIC) above CDC alert value among clinical isolates, and could be multiplexed with previously validated gyrA PCR to predict ciprofloxacin susceptibility.

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