Characterizing the Pyrenophora teres f. maculata - Barley Interaction Using Pathogen Genetics

Pyrenophora teres f. maculate is the cause of the foliar disease spot form net blotch (SFNB) on barley. To evaluate pathogen genetics underlying the P. teres f. maculate- barley interaction, we developed a 105-progeny population by crossing two globally diverse isolates, one from North Dakota, USA and the other from Western Australia. Progeny were phenotyped on a set of four barley genotypes showing a differential reaction to the parental isolates, then genotyped using a restriction-site associated - genotype by sequencing (RAD-GBS) approach. Genetic maps were developed for use in quantitative trait locus (QTL) analysis to identify virulence-associated QTL. Six QTL were identified on five different linkage groups and individually accounted for 20 to 37% of the disease variation with the number of significant QTL ranging from two to four for the barley genotypes evaluated. The data presented demonstrates the complexity of virluence involved in the P. teres f. maculate-barley interaction and begins to lay the foundation for understanding this important interaction.

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