Pepsin was used to effectively degrade chitosan in order to make it more useful in biotechnological applications. The optimal conditions of enzymolysis were investigated on the basis of the response surface methodology (RSM). The structure of the degraded product was characterized by degree of depolymerization (DD), viscosity, molecular weight, FTIR, UV-VIS, SEM and polydispersity index analyses. The mechanism of chitosan degradation was correlated with cleavage of the glycosidic bond, whereby the chain of chitosan macromolecules was broken into smaller units, resulting in decreasing viscosity. The enzymolysis by pepsin was therefore a potentially applicable technique for the production of low molecular chitosan. Additionally, the substrate degradation kinetics of chitosan were also studied over a range of initial chitosan concentrations (3.0~18.0 g/L) in order to study the characteristics of chitosan degradation. The dependence of the rate of chitosan degradation on the concentration of the chitosan can be described by Haldane's model. In this model, the initial chitosan concentration above which the pepsin undergoes inhibition is inferred theoretically to be about 10.5 g/L.
from #Medicine via ola Kala on Inoreader http://ift.tt/2979ink
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
-
▼
Ιουλίου
(489)
-
▼
Ιουλ 01
(33)
- A novel method of facial rejuvenation using a 2940...
- Laser biostimulation of wound healing: bioimpedanc...
- IJERPH, Vol. 13, Pages 672: Response to the Fernán...
- IJERPH, Vol. 13, Pages 671: Suicide: Incidence or ...
- Entropy, Vol. 18, Pages 248: Cumulative Paired φ-E...
- IJERPH, Vol. 13, Pages 666: Community-Based Manage...
- Toxins, Vol. 8, Pages 203: Relationship between Va...
- Membranes, Vol. 6, Pages 37: Efficiently Combining...
- IJERPH, Vol. 13, Pages 670: Approaches to Children...
- Sensors, Vol. 16, Pages 1016: Field Measurement-Ba...
- Energies, Vol. 9, Pages 515: Smart Monitoring Embe...
- Nutrients, Vol. 8, Pages 405: The Emerging Role of...
- Trends in ophthalmology resident surgical experien...
- Effects of anti-vascular endothelial growth factor...
- Mathematics, Vol. 4, Pages 45: Fourier Spectral Me...
- Sensors, Vol. 16, Pages 991: Development of Torque...
- Sustainability, Vol. 8, Pages 622: From Waste Mana...
- Bioengineering, Vol. 3, Pages 17: Optimization and...
- Medicines, Vol. 3, Pages 16: Necrostatin-1 Attenua...
- Hydrology, Vol. 3, Pages 25: Runoff and Soil Erosi...
- Sensors, Vol. 16, Pages 1020: Sequential Total Var...
- C, Vol. 2, Pages 18: Thermochemistry of a Biomimet...
- Reduction in respiratory motion artefacts on gadox...
- Nanomaterials, Vol. 6, Pages 127: A Gallium Oxide-...
- Administrative Sciences, Vol. 6, Pages 7: Customer...
- Local field potentials get funny
- Letter to the Editor
- Reply from Peter A. Watterson and Graham M. Nicholson
- Issue Information
- Reverse engineering inhibitory circuits in hippoca...
- Nerve regeneration in the peripheral and central n...
- Limitations of the F-wave test in monitoring spina...
- Reply from S. C. Gandevia, S. L. Khan and J. L. Ta...
-
▼
Ιουλ 01
(33)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Παρασκευή 1 Ιουλίου 2016
Bioengineering, Vol. 3, Pages 17: Optimization and Characterization of Chitosan Enzymolysis by Pepsin
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.