A rapid and ultrasensitive SERRS assay for histidine and tyrosine based on azo coupling

Publication date: 1 October 2016
Source:Talanta, Volume 159
Author(s): Huimin Sui, Yue Wang, Zhi Yu, Qian Cong, Xiao Xia Han, Bing Zhao
A simple and highly sensitive surface-enhanced resonance Raman scattering (SERRS)-based approach coupled with azo coupling reaction has been put forward for quantitative analysis of histidine and tyrosine. The SERRS-based assay is simple and rapid by mixing the azo reaction products with silver nanoparticles (AgNPs) for measurements within 2min. The limits of detection (LODs) of the method are as low as 4.33×10⁻¹¹ and 8.80×10⁻¹¹M for histidine and tyrosine, respectively. Moreover, the SERRS fingerprint information specific to corresponding amino acids guarantees the selective detection for the target histidine and tyrosine. The results from serum indicated the potential application of the proposed approach into biological samples. Compared with the methods ever reported, the main advantages of this methodology are simpleness, rapidity without time-consuming separation or pretreatment steps, high sensitivity, selectivity and the potential for determination of other molecules containing imidazole or phenol groups.

Graphical abstract

from #Medicine via ola Kala on Inoreader http://ift.tt/2912XeS
via IFTTT