MVA is an attenuated vector that still retains immunomodulatory genes. We have previously reported its optimization after deleting the C12L gene, coding for the IL-18 binding-protein. Here, we analyzed the immunogenicity of MVA vectors harboring the simultaneous deletion of A44L, related to steroid synthesis and A46R, a TLR-signaling inhibitor (MVAΔA44L-A46R); or also including a deletion of C12L (MVAΔC12L/ΔA44L-A46R). The absence of biological activities of the deleted genes in the MVA vectors was demonstrated. Adaptive T-cell responses against VACV epitopes, evaluated in spleen and draining lymph-nodes of C57Bl/6 mice at acute/memory phases, were of higher magnitude in those animals that received deleted MVAs compared to MVAwt. MVAΔC12L/ΔA44L-A46R generated cellular specific memory responses of higher quality characterized by bifunctionality (CD107a/b+/IFN-γ+) and proliferation capacity. Deletion of selected genes from MVA generated innate immune responses with higher levels of determining cytokines related to T-cell response generation, such as IL-12, IFN-γ, as well as IL-1β and IFN-β. This study describes for the first time that simultaneous deletion of the A44L, A46R and C12L genes from MVA improved its immunogenicity by enhancing the host adaptive and innate immune responses, suggesting that this approach comprises an appropriate strategy to increase the MVA vaccine potential.
from #Medicine via ola Kala on Inoreader http://ift.tt/1XMEygw
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
-
▼
Μαΐου
(630)
-
▼
Μαΐ 23
(32)
- Viruses, Vol. 8, Pages 139: Deletion of A44L, A46R...
- Monitoring Drug-Lipid Membrane Interactions via Mo...
- Preventing cancer with lifestyle counseling
- Sensors, Vol. 16, Pages 748: A Review of Distribut...
- Remote Sensing, Vol. 8, Pages 436: Hierarchical Co...
- Sensors, Vol. 16, Pages 752: An Improved Measureme...
- IJMS, Vol. 17, Pages 791: Circulating MicroRNAs as...
- Computers, Vol. 5, Pages 9: Video over DSL with LD...
- IJMS, Vol. 17, Pages 792: A Novel Technique to Det...
- Remote Sensing, Vol. 8, Pages 435: Impact of Satel...
- Viruses, Vol. 8, Pages 145: Experimental Infection...
- IJMS, Vol. 17, Pages 795: Cold-Induced Browning Dy...
- IJMS, Vol. 17, Pages 771: Analysis of 2-(2-Phenyle...
- IJMS, Vol. 17, Pages 795: Cold-Induced Browning Dy...
- IJMS, Vol. 17, Pages 791: Circulating MicroRNAs as...
- IJMS, Vol. 17, Pages 793: The Role of Proanthocyan...
- IJMS, Vol. 17, Pages 794: Gibberellic Acid: A Key ...
- IJMS, Vol. 17, Pages 793: The Role of Proanthocyan...
- IJMS, Vol. 17, Pages 794: Gibberellic Acid: A Key ...
- Symmetry, Vol. 8, Pages 37: Optional Frame Selecti...
- IJERPH, Vol. 13, Pages 520: Antifungal Effect of H...
- Atmosphere, Vol. 7, Pages 73: Composition and Sour...
- Water, Vol. 8, Pages 216: Opening the Black Box: U...
- IJMS, Vol. 17, Pages 771: Analysis of 2-(2-Phenyle...
- Biology, Vol. 5, Pages 22: Effect of Different Bro...
- Molecules, Vol. 21, Pages 679: New Casbane and Cem...
- Steamed and Fermented Ethanolic Extract from Codon...
- Implementing Pharmacogenomics at Your Institution:...
- Cancers, Vol. 8, Pages 52: The MYC 3′ Wnt-Responsi...
- Atmosphere, Vol. 7, Pages 71: The Effect of Season...
- Coatings, Vol. 6, Pages 21: The Acoustical Durabil...
- Metals, Vol. 6, Pages 122: Hydrometallurgy
-
▼
Μαΐ 23
(32)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Δευτέρα 23 Μαΐου 2016
Viruses, Vol. 8, Pages 139: Deletion of A44L, A46R and C12L Vaccinia Virus Genes from the MVA Genome Improved the Vector Immunogenicity by Modifying the Innate Immune Response Generating Enhanced and Optimized Specific T-Cell Responses
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.