Production and characterization of a new glycolipid, mannosylerythritol lipid, from waste cooking oil biotransformation by Pseudozyma aphidis ZJUDM34

The production of MELs using waste cooking oil instead of soybean oil was successful, and the major characteristics and compositions of MELs were also investigated. The use of inexpensive fermentation substrates associated with straightforward downstream processing is expected to have a great impact on the economy of MEL production.

Abstract

Mannosylerythritol lipids (MELs) are glycolipids possessing unique biosurfactant properties. However, the prices of substrates currently used for MEL formation caused its unsustainable commercial development. Waste cooking oil poses significant ecological and economical problems. Thus, the production of MELs from used waste cooking oil using the biotransformation route is one of the better alternatives to utilize it efficiently and economically. This work aims at the production of MELs using waste cooking oil instead of soybean oil and evaluating the major characteristics and compositions of MELs. The titers reached 61.50 g/L by the optimization of culture medium, higher than the counterpart (10.25 ± 0.32 g/L) of the nonoptimized medium. MELs exhibited good surface activity and better performance in contrast to MELs grown on soybean oil. The water phase behavior of MEL‐A was also evaluated. The process showed higher productivity of MELs with better surface activity and application stability than the conventional process using soybean oil. The findings of this study imply that the use of inexpensive fermentation substrates associated with straightforward downstream processing is expected to have a great impact on the economy of MEL production.

https://onlinelibrary.wiley.com/doi/abs/10.1002/fsn3.880?af=R