Bovine teeth can reliably substitute human dentine in an intra‐tooth push‐out bond strength model?

Abstract

Aim

To investigate the reliability of using bovine teeth as an alternative to human teeth in an intra‐tooth push‐out model by comparing the bond strength of three root canal sealers in both tooth substrates.

Methodology

From human and bovine incisors (12 per group), one 1‐mm disc was obtained from each root. On each disc, three standard 0.8mm‐diameter holes were drilled through the dentine. After irrigation, the holes were filled with one of the three tested root canal sealers: AH Plus, MTA Fillapex or Total Fill BC Sealer. Tooth slices were kept in contact with sterile gauze moistened in PBS solution (pH=7.2) for 7 days at 37°C, and finally, a push‐out load was applied until sealer dislocation. The bonded interface area was calculated to obtain data in MPa. The push‐out data for each sealer and the overall push‐out data for human and bovine teeth were compared (Mann‐Whitney U). Kruskal‐Wallis was used to study the sealers behavior at each of the dental substrates. Multiple comparisons were performed using Mann‐Whitney U after Bonferroni correction (P<0.05).

Results

Dentine substrate did not influence the push‐out of either, individual sealers or overall sealers tested (Mann‐Whitney U,P>0.05). In both human and bovine samples, AH Plus performed better than BC Sealer and MTA Fillapex, while BC Sealer also provided a superior push‐out compared to MTA Fillapex (Mann‐Whitney U, P<0.05).

Conclusion

When using an intra‐tooth model, bovine teeth do not significantly influence the bond strength of sealers.

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