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Τετάρτη 28 Νοεμβρίου 2018

ANALYSIS OF CELL VIABILITY AND GENE EXPRESSION AFTER CONTINUOUS ULTRASOUND THERAPY IN L929 FIBROBLAST CELLS

Objective This study aimed to analyze cell viability and gene expression IL-6 (interleukin-6) and VEGF (vascular endothelial growth factor) after continuous ultrasound therapy of 1 and 3 MHz on L929 fibroblast cells. Design The L929 cells were cultivated in 12-well plates and divided into 5 groups: G1: non-irradiated, G2: 0.2 W/cm2-1 MHz, G3: 0.5 W/cm2-1 MHz, G4: 0.2 W/cm2-3 MHz and G5: 0.5 W/cm2-3 MHz. The cells were irradiated at 24 hours and 48 hours. Cell viability was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The gene expression analysis was assessed using real-time PCR. Results The G2 and G3 groups showed a decrease in cell viability when compared to the G1 at 48 hours (p

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