Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.
https://ift.tt/2NiwPpl
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
▼
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
-
▼
Αυγούστου
(5863)
-
▼
Αυγ 25
(98)
- Researching health in diverse neighbourhoods: crit...
- Below normal birth weight in the Northwest part of...
- Intestinal parasitosis among HIV/AIDS patients who...
- Memantine has no effect on KATP channels in pancre...
- How did cyanobacteria first embark on the path to ...
- Steep redox gradient and biogeochemical cycling dr...
- A potential central role of Thaumarchaeota in N-Cy...
- Antioxidant and Anticancer Roles of a Novel Strain...
- Most Research Participants Not Concerned About Dat...
- Characterization of the symptoms of neurogenic ort...
- Fusing multi-scale information in convolution netw...
- Not again! Effect of previous test results, age gr...
- A 24-Year-Old Man with Chronic Constipation and Ac...
- Integrating Patient-reported Outcomes Into Treat t...
- Letter to Editor regarding CGH 2018; 16:1064-72
- Re: Houston Consensus Conference on Testing for He...
- The Gut Microbiome in Adult and Pediatric Function...
- Refining Sample-Size Estimations Based Upon Placeb...
- Peroral Endoscopic Myotomy is Effective for Patien...
- The Who and Why of Herpes Zoster Vaccination in Pa...
- Long-term outcomes of combined endoscopic resectio...
- Cerebrospinal Fluid Hyaluronan and Neurofibromatos...
- Analysis of Epididymal Protein Synthesis and Secre...
- The Swapping Constraint
- The Role of Observers in Computations
- Tick Microbiome Characterization by Next-Generatio...
- Endogenous Protein Tagging in Human Induced Plurip...
- Generation of Electronic Cigarette Aerosol by a Th...
- A Flexible Low Cost Hydroponic System for Assessin...
- A Simplified and Efficient Method to Isolate Prima...
- Neuroprotective exendin-4 enhances hypothermia the...
- Parkinsonian signs in patients with cervical dysto...
- Balance control systems in Parkinson’s disease and...
- Organotypic Culture Method to Study the Developmen...
- EMCrit 231 – How to Practice Cricothyroidotomy (Cric)
- Measuring Biodiversity and Extinction – Present an...
- Predictors of a successful external cephalic versi...
- A cross-country survey of attitudes toward childbi...
- What attributes do Australian midwifery leaders id...
- One-Year Outcomes after PCI Strategies in Cardioge...
- Crystallizing Galactocele: A rare entity—report of...
- Role of FNAC in diagnosing lesions of hand and wrist
- Novel cytomorphologic characteristics suggesting h...
- Cytologic diagnosis of adenocarcinoma on bile duct...
- Parotid gland chondroma masquerading as pleomorphi...
- Low Back Pain in Persons with Lower Extremity Ampu...
- Burkholderia pseudomallei-absent soil bacterial co...
- Biotransformation of vanillin into vanillyl alcoho...
- Abnormal event detection in crowded scenes using h...
- NRAS Q61K mutated diffuse leptomeningeal melanomat...
- Circulating tumor cells and cell-free nucleic acid...
- Enhanced Cancer Vaccination by In Situ Nanomicelle...
- Effect of combination of pre- and postoperative pu...
- The Role of Oncolytic Viruses in the Treatment of ...
- The assessment of the esophageal motility of child...
- Circumferential distribution and clinical characte...
- Prior exposure of pancreatic tumors to [sorafenib ...
- Cancers, Vol. 10, Pages 289: miRNA-205 Nanoformula...
- Epithelial‐mesenchymal transition is activated in ...
- Elevation of Androgen Receptor Promotes Prostate C...
- Novel therapeutic strategies for advanced ovarian ...
- Utility of the Quasi‐monomorphic Variation Range i...
- BPR1J373, a novel multi‐targeted kinase inhibitor,...
- Knockdown of LGALS12 inhibits porcine adipocyte ad...
- TGF-β signaling in cancer
- Evaluation of a smartphone photoscreening app to d...
- New corneal assessment index from the relational t...
- Blood pressure, ocular perfusion pressure and open...
- Real-life results of switching from preserved to p...
- Risk factors for early onset elevated intraocular ...
- Further evidence for a differential interaction of...
- Psychiatric comorbidity and traumatic brain injury...
- Techniques for placement of stereotactic electroen...
- The use of antidepressant drugs in pregnant women ...
- Design and synthesis of 5‐(5‐nitrothiophen‐2‐yl)‐3...
- Extending the developmental origins of disease mod...
- Issue Information
- Tumor‐shed antigen CA125 blocks complement‐mediate...
- Eomes controls the development of Th17‐derived (no...
- RESPONDER – diagnosis of pathological complete res...
- When Enough Is Not Enough: Screening, Brief Interv...
- Long‐term results of osseointegrated implant‐based...
- Long‐term stability of autologous bone graft of in...
- Histone Lysine and Genomic Targets of Histone Acet...
- Acute Effects of Meditation Training on the Waking...
- Modulating auditory selective attention by non‐inv...
- Targeting Redox Regulation to Treat Substance Use ...
- Female genital mutilation and non‐invasive cervica...
- BRAFV600E mutation: A promising target in colorect...
- Patient Reported Outcome Measures (PROMs) of poste...
- Exposing {010} Active Facets by Multiple‐Layer Ori...
- Abnormal Near‐Infrared Absorption in 2D Black Phos...
- Protein‐Corona‐by‐Design in 2D: A Reliable Platfor...
- Bile acid homeostasis and intestinal dysbiosis in ...
- Histologic Features and Prognostic Significance of...
- Novel EPC1 gene fusions in endometrial stromal sar...
- Correction to: Ileocolic intussusception due to a ...
- Per-oral Pyloromyotomy (POP) for Medically Refract...
-
▼
Αυγ 25
(98)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Σάββατο 25 Αυγούστου 2018
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.