[ 11 C]SCH23390 binding to the D 1 -dopamine receptor in the human brain—a comparison of manual and automated methods for image analysis

Abstract

Background

The D₁-dopamine receptor radioligand [¹¹C]SCH23390 has been frequently used in PET studies. In drug-naïve patients with schizophrenia, the findings have been inconsistent, with decreases, increases, and no change in the frontal cortex D₁-dopamine receptors. While these discrepancies are likely primarily due to a lack of statistical power in these studies, we speculated that an additional explanation may be the differences due to methods of image analysis between studies, affecting reliability as well as bias between groups.

Methods

Fifteen healthy subjects underwent two PET measurements with [¹¹C]SCH23390 on the same day. The binding potential (BP_ND) was compared using a 95% confidence interval following manual and automated delineation of a region of interest (ROI) as well as with and without frame-by-frame realignment.

Results

Automated target region delineation produced lower BP_ND values, while automated delineation of the reference region yielded higher BP_ND values. However, no significant differences were observed for repeatability using automated and manual delineation methods. Frame-by-frame realignment generated higher BP_ND values and improved repeatability.

Conclusions

The results suggest that the choice of ROI delineation method is not an important factor for reliability, whereas the improved results following movement correction confirm its importance in PET image analysis. Realignment is therefore especially important for measurements in patient populations such as schizophrenia or Parkinson's disease, where motion artifacts may be more prevalent.

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