The co-identification of mcr-4.6 and blaNDM-1 in a clinical Enterobacter cloacae isolate from China [PublishAheadOfPrint]

Here we describe the first report of a clinical colistin-resistant ST84 Enterobacter cloacae isolate co-harboring mcr-4.6 (previously named mcr-4.2) and bla_NDM-1 from a patient in China. The bla_NDM-1-harboring IncX3 plasmid and the novel mcr-4.6-harboring ColE plasmid were completely sequenced. Although this isolate showed high level resistance to colistin, mcr-4.6 plasmid transformation, gene subcloning, susceptibility testing and lipid A matrix-assisted laser desorption ionization mass spectrometry analysis indicate that mcr-4.6 itself doesn't confer resistance to colistin.

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