Spatiomechanical modulation of EphB4:ephrin-B2 signaling in neural stem cell differentiation

Interactions between EphB4 receptor tyrosine kinases and their membrane-bound ephrin-B2 ligands on apposed cells play a regulatory role in neural stem cell differentiation. With both receptor and ligand constrained to move within the membranes of their respective cells, this signaling system inevitably experiences spatial confinement and mechanical forces in conjunction with receptor-ligand binding. In this study, we reconstitute the EphB4:ephrin-B2 juxtacrine signaling geometry using a supported lipid bilayer system presenting laterally mobile and monomeric ephrin-B2 ligands to live neural stem cells.

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