PET-PCR for Detection of Plasmodium falciparum Plasmepsin 2 Gene copy number [PublishAheadOfPrint]

Piperaquine is an important partner drug used in artemisinin-based combination therapies (ACTs). An increase in the plasmepsin 2 and 3 gene copy number has been associated with decreased susceptibility of Plasmodium falciparum to piperaquine in Cambodia. Here, we developed a photo- induced electron transfer real-time PCR (PET-PCR) assay to quantify the copy number of plasmepsin 2 (PfPM2) that can be used in endemic countries to enhance molecular surveillance.

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