Outcome analysis in Crohn’s disease using endomicroscopy: How important and reliable is in vivo analysis of microscopic changes?

Endomicroscopy provides in vivo histologic analysis, functional imaging, and molecular imaging. Endomicroscopy is a unique technology, which provides in vivo histologic analysis during ongoing endoscopy. Endomicroscopy has been shown to be highly effective in defining final histologic features if used in experienced hands.1 However, there are several limitations of the technology. The grade of dysplasia cannot be assessed properly because fluorescein (a commonly used contrast agent in endomicroscopy) does not label nuclei.

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