Preparation and evaluation of effect on E. coli and S. aureus of radiolabeled Ampicillin loaded graphene oxide nanoflakes

Abstract

Ampicillin is a one of effective antibiotics against Gram-positive and Gram-negative bacteria. The present study aims to label ampicillin loaded graphene oxide nanoflake (AMP-GO) with ^99mTc and evaluate of its in vitro binding to S. aureus and E. coli. Firstly, ampicillin was loaded onto graphene oxide nanoflake prepared. AMP-GO was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM) techniques, and the amount of loaded ampicillin onto GO was determined by UV-Vis absorption spectroscopy. AMP and AMP-GO were labeled with ^99mTc using stannous chloride reducing agent. Labeling efficiency of ^99mTc-AMP-GO was found to be 97.66±2.06%. ^99mTc-AMP-GO has higher binding efficiencies to both S. aureus and E. coli than ^99mTc-AMP. ^99mTc-AMP-GO could be promising candidate as agent infection nuclear imaging. Furthermore in vivo studies of ^99mTc-AMP-GO with infected rats are planned to be done.

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Ampicillin-Graphene oxide (AMP-GO) nanoparticles by evaluating their in vitro binding in S. aureus and E. coli for infection nuclear imaging.

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