High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

Here, we present a protocol to image human pancreas sections in three dimensions (3D) using optimized passive clearing methods. This manuscript demonstrates these procedures for passive optical clearing followed by multiple immunofluorescence staining to identify key elements of the autonomic and sensory neural networks innervating human islets.

http://ift.tt/2DIqUFb