Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

This protocol describes how to study cellular processes during cell fate conversion in Caenorhabditis elegans in vivo. Using transgenic animals,allowing heat-shock promoter-driven overexpression of the neuron fate-inducing transcription factorCHE-1 and RNAi-mediated depletion of the chromatin-regulating factor LIN-53germ cell to neuron reprogramming can be observed in vivo.

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