Expression profile analysis of long noncoding RNA in Acute Myeloid Leukemia by microarray and bioinformatics

Abstract

Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides, which are involved in tumorigenesis and play a key role in cancer progression. To determine whether lncRNAs are involved in acute myeloid leukemia (AML), we analyzed the expression profile of lncRNAs and mRNAs in AML. 5 pairs of AML patients and iron deficiency anemia (IDA) controls were screened by microarray. Through co-expression analysis, differently expressed transcripts were done into modules, and lncRNAs got the functional annotations. We further analyzed the clinical significance of crucial lncRNAs from the modules in the public data. Finally, the expression of 3 lncRNAs, RP11-222K16.2, AC092580.4 and RP11-305O.6 were validated differently in newly diagnosed AML, AML replase and IDA by qRT-PCR, which may be associated with AML patients' overall survival. Further analysis showed that RP11-222K16.2 might affect the differentiation of NK cells, and promote the immunized evasion of AML by regulating Eomesodermin (Eomes) expression. Analysis of this study revealed that dysregulated lncRNAs and mRNAs in AML versus IDA controls that could affect immune system and hematopoietic cell differentiation. Those lncRNA's biological functions need to be further validated.

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