Measuring the Invisible: Determining the Size of Growing Nanodomains Using the “Inverse FCS”

Molecular recruitment into functional domains in the cell plasma membrane is an important prerequisite for many cellular processes, such as cell signaling, cell-matrix adhesion, or the formation of synapses. High precision measurements of the origin and evolution of these structures is key to their understanding; however, experimental methods with which one can detect molecular dynamics and aggregation, with microsecond temporal resolution and at nanometer scales, are scarce (1–4). One of the most developed approaches, which is up to the task, is fluorescence correlation spectroscopy (FCS).

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