Abstract
Purpose
To develop a robust ECM and enhance cellularmaterial interaction, we developed a cysteine-based nanocomposite combined with chitosan, nanohydroxyapatite and gelatin (cysteine-CnHAG) to effectuate essential factors.
Methods
The scaffolds were fabricated under same conditions using a freeze-drying technique and characterized with a scanning electron microscopy to study its internal architecture including pore distribution. They were further interacted with human bone marrow derived mesenchymal stem cells (hMSCs) to investigate its biocompatibility. In this, cell viability and cell adhesion was assessed using optical microscopy.
Results
From the results, the scaffolds display a non-toxic effect with hMSCs and have a pore size range of between 12-142 μm. The result of the optical microscopy showed adhesion of the cells on the scaffold matrix of both cysteine- CnHAG and CnHAG, which implies the microenvironment of the scaffold is largely suitable for the MSC development
Conclusions
In conclusion, we believe cysteine-CnHAG can be a novel nanocomposite for potential applications in the field of bone tissue engineering.
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