Molecular Confirmation of Conserved Nature of Genes Encoding MPB53 and MPB63 Immune Dominant Proteins in Indian Strain of Mycobacterium bovis

Abstract

The genes encoding MPB53 and MPB63 immuno dominant proteins of Mycobacterium bovis field strain 3/86-RV were amplified by polymerase chain reaction (PCR). The recombinant plasmids were constructed via inserting the mpb53 and mpb63 genes into the pET vector (named pET28b-mpb53 and pET28b-mpb63). The nucleotide sequences of the insert PCR products were determined by vector specific primer. Both genes contained an open reading frame of 402 and 390 base pairs for mpb53 and mpb63, respectively. The sequenced genes and its deduced amino acid sequences were compared with the published sequences of reference M. tuberculosis complex strains. The sequences of the mpb53 and mpb63 genes share more than 99 % nucleotide homology and deduced protein sequence homology pointing towards conserved molecular nature of these immunodominant proteins among M. tuberculosis complex species.

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