Roseovarius lacus sp. nov., isolated from Yuncheng Saline Lake, China

Abstract

Strain GSS12^T, a Gram-negative, aerobic, non-flagellated, ovoid- to rod-shaped (0.5–0.7 × 0.9–3.0 µm) bacterium, was isolated from Yuncheng Saline Lake, China. Growth occurred with 0.5–16.0 % (w/v) NaCl (optimum 4.5 %), at pH 5.0–10.0 (optimum pH 6.0–6.5) and at 10–50 °C (optimum 37 °C). The major fatty acids (>5.0 %) found in GSS12^T were summed feature 8 (72.2 %), C_16:0 (9.0 %) and C_18:1ω7c 11-methyl (6.4 %). The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequences showed that strain GSS12^T forms a stable clade with species of the genus Roseovarius, being related to R. pacificus 81-2^T and R. litoreus GSW-M15^T with 97.9 and 96.7 % of sequence similarity, respectively. The DNA–DNA relatedness values between strain GSS12^T and R. pacificus 81-2^T and R. halotolerans HJ50^T were low (36 and 29 %, respectively). The phenotypic, physiological, biochemical and genetic characteristics support the assignment of strain GSS12^T to the genus Roseovarius and represent a novel species. The name Roseovarius lacus sp. nov. is proposed, with strain GSS12^T (=KCTC 52185^T =MCCC 1K02302^T) as the type strain.

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