Publication date: 17 August 2016
Source:Vaccine, Volume 34, Issue 37
Author(s): M.E. Hoonakker, L.M. Verhagen, L. van der Maas, B. Metz, J.P. Uittenbogaard, B. van de Waterbeemd, C.A.C.M. van Els, W. van Eden, C.F.M. Hendriksen, A. Sloots, W.G.H. Han
Whole cell Bordetella pertussis (wP) vaccines are still used in many countries to protect against the respiratory disease pertussis. The potency of whole-cell pertussis vaccine lots is determined by an intracerebral challenge test (the Kendrick test). This test is criticized due to lack of immunological relevance of the read-out after an intracerebral challenge with B. pertussis. The alternative in vivo test, which assesses specific antibody levels in serum after wP vaccination, is the Pertussis Serological Potency test (PSPT). Although the PSPT focuses on a parameter that contributes to protection, the protective immune mechanisms after wP vaccination includes more elements than specific antibody responses only. In this study, additional parameters were investigated, i.e. circulating pro-inflammatory cytokines, antibody specificity and T helper cell responses and it was evaluated whether they can be used as complementary readout parameters in the PSPT to assess wP lot quality. By deliberate manipulation of the vaccine preparation procedure, a panel of high, intermediate and low quality wP vaccines were made. The results revealed that these vaccines induced similar IL-6 and IP10 levels in serum 4h after vaccination (innate responses) and similar antibody levels directed against the entire bacterium. In contrast, the induced antibody specificity to distinct wP antigens differed after vaccination with high, intermediate and low quality wP vaccines. In addition, the magnitude of wP-induced Th cell responses (Th17, Th1 and Th2) was reduced after vaccination with a wP vaccine of low quality. T cell responses and antibody specificity are therefore correlates of qualitative differences in the investigated vaccines, while the current parameter of the PSPT alone was not sensitive enough to distinguish between vaccines of different qualities. This study demonstrates that assessment of the magnitude of Th cell responses and the antigen specificity of antibodies induced by wP vaccination could form valuable complementary parameters to the PSPT.
http://ift.tt/2adH8c7
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
-
▼
Ιουλίου
(489)
-
▼
Ιουλ 28
(51)
- Sulforaphene–Carboplatin Combination Synergistical...
- Uncoupling Mitochondrial Respiration for Diabesity
- Uncoupling Mitochondrial Respiration for Diabesity
- Reliability and Responsiveness of NutriQoL ® Quest...
- A novel and label-free immunosensor for bisphenol ...
- Silicone rubber selection for passive sampling of ...
- Ringer tablet-based ionic liquid phase microextrac...
- FTIR and NDIR spectroscopies as valuable alternati...
- Liquid-phase microextraction of organophosphorus p...
- A nanoparticulate liquid binding phase based DGT d...
- The development of a quantitative and qualitative ...
- Sensitive QD@SiO2-based immunoassay for triplex de...
- Surface-enhanced Raman spectroscopy (SERS) in food...
- Self-assembled cyclodextrin-modified gold nanopart...
- Biosensing applications of titanium dioxide coated...
- Carbon nanotube sponges as a solid-phase extractio...
- Application of a new version of GA-RBF neural netw...
- Aptamer contained triple-helix molecular switch fo...
- An ELISA method detecting the active form of suPAR
- Identifying the tobacco related free radicals by U...
- Hierarchical Graphene coating for highly sensitive...
- Electrochemically reduced graphene oxide-modified ...
- A multicommutated tester of bioreactors for flow a...
- Effective immobilization of tyrosinase via enzyme ...
- The effect of lengthening contractions on neuromus...
- Isolated Fibular Fracture - Everything You Need To...
- Dynamic Modulation of DNA Hybridization Using Allo...
- High-Throughput Optical Sensing Immunoassays on Sm...
- Simultaneous UV–Visible Absorption and Raman Spect...
- Measuring indirect effects of rotavirus vaccine in...
- Gavi’s policy steers country ownership and self-fi...
- The safety and reactogenicity of a reduced-antigen...
- Meningococcal pneumonia
- Disparities in PCV impact between different ethnic...
- Editorial Board/Aims and Scope
- Strain-dependent and distinctive T-cell responses ...
- The role of travel in measles outbreaks in Austral...
- Two potential recombinant rabies vaccines expressi...
- A DNA vaccine encoding VP22 of herpes simplex viru...
- Adverse events following pandemic influenza A (H1N...
- Concordance of adolescent human papillomavirus vac...
- Quantitative and epitope-specific antigenicity ana...
- Adaptive immune response to whole cell pertussis v...
- Vaccine-preventable disease-related hospitalizatio...
- Safety of the 2010–11, 2011–12, 2012–13, and 2013–...
- Safety of the 2011–12, 2012–13, and 2013–14 season...
- The Three False Dawns of Biomedical Gerontology
- Secretome profiling of apheresis platelet supernat...
- A combined proteomic and transcriptomic analysis o...
- Bladder perforation by ventriculoperitoneal shunt
- Primary pulmonary myxoma
-
▼
Ιουλ 28
(51)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.