Publication date: Available online 6 June 2016
Source:Vaccine
Author(s): Rosa Collado, Antoni Prenafeta, Luis González-González, Josep Antoni Pérez-Pons, Marta Sitjà
Streptococcus uberis is a worldwide pathogen that causes intramammary infections in dairy cattle. Because virulence factors determining the pathogenicity of S. uberis have not been clearly identified so far, a commercial vaccine is not yet available. Different S. uberis strains have the ability to form biofilm in vitro, although the association of this kind of growth with the development of mastitis is unknown. The objective of this study was to evaluate the potential use as vaccine antigens of proteins from S. uberis biofilms, previously identified by proteomic and immunological analyses. The capability of eliciting a protective immune response by targeted candidates was assayed on a murine model. Sera from rabbits immunized with S. uberis biofilm preparations and a convalescent cow intra-mammary infected with S. uberis were probed against cell wall proteins from biofilm and planktonic cells previously separated by two-dimensional gel electrophoresis. Using rabbit immunized serum, two proteins were found to be up-regulated in biofilm cells as compared to planktonic cells; when serum from the convalescent cow was used, up to sixteen biofilm proteins were detected. From these proteins, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), fructose-biphosphate aldolase (FBA), and elongation factor Ts (EFTs) were chosen to be tested as vaccine antigen candidates. For this purpose, different groups of mice were immunized with the three recombinant-expressed proteins (each one formulated separately in a vaccine), and thereafter intraperitoneally challenged with S. uberis. The three proteins induced specific IgG antibodies, but a significant reduction of mortality was only observed in the groups of mice vaccinated with FBA or EFTs. These results suggest that FBA and EFTs might be considered as strong antigenic candidates for a vaccine against S. uberis bovine mastitis. Moreover, this is the first study to indicate that also in S. uberis, GAPDH, FBA and EFTs, as proteins detected in both cytoplasm and cell wall fractions, can play a second function (moonlighting), the latter being particularly involved in the virulence of such a pathogen organism.
from #Medicine via ola Kala on Inoreader http://ift.tt/1ra4dCx
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
-
▼
Ιουνίου
(946)
-
▼
Ιουν 06
(83)
- Ceramic variation and negotiated communities in th...
- Electronic nicotine delivery system landscape in l...
- Validation of the Kinyarwanda-version Short-Form L...
- Default options in advance directives: study proto...
- Identifying improvements to complex pathways: evid...
- When procedures meet practice in community pharmac...
- Clinical outcomes at 12 months and risk of inflamm...
- Comparing cognitive-behavioural psychotherapy and ...
- Longitudinal trends in use and costs of targeted t...
- Effect of affordable technology on physical activi...
- HIV infection and bone disease
- Endothelial Progenitor Cells Predict Long-Term Mor...
- Exosomes Mediate the Intercellular Communication a...
- Differential microRNA Profiles Predict Diabetic Ne...
- Palmitate-induced Regulation of PPARγ via PGC1α: a...
- The Association of -429T>C and -374T>A Polymorphis...
- Inverse Association between Sodium Channel-Blockin...
- Longitudinal Associations between Triglycerides an...
- Dietary Flaxseed Mitigates Impaired Skeletal Muscl...
- Gender Related Survival Differences in ST-Elevatio...
- Melissa Officinalis L. Extracts Protect Human Reti...
- Study and Therapeutic Progress on Intracranial Ser...
- Impact of JAK2V617F Mutation Burden on Disease Phe...
- Detection of Human Papillomavirus 16-Specific IgG ...
- The Prodomain-Containing BMP9 Produced from a Stab...
- Daclatasvir plus Asunaprevir Treatment for Real-Wo...
- Dynamic Compression Effects on Immature Nucleus Pu...
- The Expression of Gli3 and Teashirt3 in the Stenot...
- A Prospective Randomized Experimental Study to Inv...
- ES140 Flexible Forceps
- ES201 Compact LED Light Source
- Sampling Error: Impact on the Quantitative Analysi...
- Contents
- Editorial Board
- Application of Two-Dimensional Nuclear Magnetic Re...
- Association between gait characteristics and endot...
- Isolation and Expansion of Mesenchymal Stem/Stroma...
- A Protocol for Collecting and Constructing Soil Co...
- Germline development in amniotes: A paradigm shift...
- Defining heterogeneity within bacterial population...
- Design of trials for interrupting the transmission...
- The impact of albendazole treatment on the inciden...
- Daily Life Negative Mood and Exhaled Nitric Oxide ...
- Loss- and Gain-of-function Approach to Investigate...
- Competitive Assays of Label-Free DNA Hybridization...
- Probing vaccine antigens against bovine mastitis c...
- Échoendoscopie et coloscopie en France : formation...
- Mobile phones and seizures: drug-resistant epileps...
- Observation of the efficacy of radiofrequency cath...
- The chemical biology of protein hydropersulfides: ...
- IJGI, Vol. 5, Pages 86: 3-Dimensional Modeling and...
- Sustainability, Vol. 8, Pages 536: Dynamic Ecologi...
- IJGI, Vol. 5, Pages 85: Comparative Perspective of...
- Marine Drugs, Vol. 14, Pages 108: Enzymatic Hydrol...
- Efficiencies of resonant and nonresonant multiphot...
- Processes, Vol. 4, Pages 19: A Review of Dynamic M...
- IJERPH, Vol. 13, Pages 565: Childhood Health Statu...
- Sensitive fluorescence assay of organophosphorous ...
- Monolith dip-it: a bifunctional device for improvi...
- Peritoneal dissemination in early gastric cancer: ...
- Effectiveness of Sofosbuvir, Ledipasvir/Sofosbuvir...
- Comparing health care utilization among three trea...
- Similar fecal microbiota signatures in patients wi...
- Is preoperative distinction between complicated an...
- Phase I study of lenalidomide and sorafenib in pat...
- Outcome of hypovascular hepatic nodules with posit...
- Real-world sustained virologic response rates of s...
- Survival outcome of salvage hepatectomy in patient...
- Hepatitis E virus (HEV) ORF2 antigen levels differ...
- Systemic inflammatory markers as prognostic factor...
- Cost-effectiveness of doxorubicin-eluting beads ve...
- Low serum vitamin D during remission increases ris...
- Randomized controlled trial of prophylactic negati...
- Clinical outcomes of pancreatic ductal adenocarcin...
- Combined medical and surgical approach improves he...
- Elucidation of the metabolic network of Helicobact...
- High fecal IgA is associated with reduced Clostrid...
- IJERPH, Vol. 13, Pages 564: Nutrient Intakes in Ea...
- Veterinary Sciences, Vol. 3, Pages 10: Sequence In...
- Dentistry Journal, Vol. 4, Pages 19: iPS Cells—The...
- OtoRhinoLaryngology & Medicine,New Articles,June 6...
- Metabolites, Vol. 6, Pages 18: Detection of Volati...
- Data, Vol. 1, Pages 7: A MODIS/ASTER Airborne Simu...
-
▼
Ιουν 06
(83)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.