Αρχειοθήκη ιστολογίου

Αναζήτηση αυτού του ιστολογίου

Παρασκευή 27 Μαΐου 2016

Structural considerations on the selectivity of an immunoassay for sulfamethoxazole

Publication date: 1 September 2016
Source:Talanta, Volume 158
Author(s): Holger Hoffmann, Stefanie Baldofski, Kristin Hoffmann, Sabine Flemig, Carla Patrícia Silva, Valdemar I. Esteves, Franziska Emmerling, Ulrich Panne, Rudolf J. Schneider
Sulfamethoxazole (SMX), a sulfonamide, is a widely used bacteriostatic antibiotic and therefore a promising marker for the entry of anthropogenic pollution in the environment. SMX is frequently found in wastewater and surface water. This study presents the production of high affinity and selective polyclonal antibodies for SMX and the development and evaluation of a direct competitive enzyme-linked immunosorbent assay (ELISA) for the quantification of SMX in environmental water samples. The crystal structures of the cross-reacting compounds sulfamethizole, N4-acetyl-SMX and succinimidyl-SMX were determined by x-ray diffraction aiming to explain their high cross-reactivity. These crystal structures are described for the first time. The quantification range of the ELISA is 0.82–63µg/L. To verify our results, the SMX concentration in 20 environmental samples, including wastewater and surface water, was determined by ELISA and tandem mass spectrometry (MS/MS). A good agreement of the measured SMX concentrations was found with average recoveries of 97–113% for the results of ELISA compared to LC-MS/MS.

Graphical abstract

image


from #Medicine via ola Kala on Inoreader http://ift.tt/25qehdz
via IFTTT

Δεν υπάρχουν σχόλια:

Δημοσίευση σχολίου

Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.