Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression

Publication date: June 2016
Source:Data in Brief, Volume 7
Author(s): Claudia Carissimi, Teresa Colombo, Gianluca Azzalin, Emanuela Cipolletta, Ilaria Laudadio, Giuseppe Macino, Valerio Fulci
We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1]). Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction as well as in Jurkat control cells lines. A parallel immunoprecipitation using isotype-matched rat IgG was performed as a control. AGO2 associated mRNAs were profiled by microarray (GEO: GSE37212). AGO2 bound miRNAs were profiled by RNA-seq.



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